FastQCFastQC Report
Mon 6 Feb 2017
SRR2077175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534993
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.039.4678841
GTATCAA57850.035.848151
TATCAAC75050.027.487372
ATCAACG75850.027.1963833
TCAACGC76250.026.9184444
CAACGCA78300.025.9497155
AACGCAG79750.025.2623546
ACGCAGA91300.021.8583747
AACGCGG800.00295957421.4875496
CGCAGAG93150.021.218928
GTGGTAT14000.020.6349751
GAGTACT67900.020.60047312
TGGTATC13100.020.4717962
CGATCGT850.003984391720.41305742
AGAGTAC91750.020.22740711
AGTACTT71550.019.69367613
CAGAGTA97200.019.55484210
GCAGAGT102700.018.9434289
GTACTTT74250.018.80204614
CGTCGTT1150.001068150518.10513346
Produced by FastQC (version 0.11.5)