FastQCFastQC Report
Mon 6 Feb 2017
SRR2077174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541359
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.035.2640461
GTATCAA41250.035.2603951
TCAACGC51500.027.5486984
CAACGCA53100.026.9136335
ATCAACG52850.026.8471153
TATCAAC54450.026.3310662
AACGCAG56000.025.5193886
ACGCAGA65000.021.7204067
GTACATG68850.020.8752081
CGCAGAG66950.020.6249568
TACATGG69850.019.6376572
AGAGTAC65650.019.06859811
ACATGGG71950.018.4727153
GCAGAGT71550.017.9977059
CAGAGTA71200.017.81385610
GAGTACT36400.017.1480812
TATACTG9150.016.9768835
CATGGGG51100.016.8882884
TTAACGC2057.716935E-716.8388584
GTACTTT39450.016.26131214
Produced by FastQC (version 0.11.5)