FastQCFastQC Report
Mon 6 Feb 2017
SRR2077174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541359
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.043.0057831
GTATCAA58050.037.263911
TATCAAC76350.027.9230422
TCAACGC75650.027.5450634
ATCAACG76450.027.5266913
CGCGTAA756.30459E-527.51032610
CAACGCA77000.026.928165
AACGCAG80000.025.9183526
ACGCAGA93600.022.0650587
CGCAGAG95950.021.2898648
GTACATG67700.019.4558431
AGAGTAC94000.019.35043711
GAGTACT64700.019.13203612
CAGAGTA97900.019.0731710
GCAGAGT101850.018.806529
TACATGG68050.018.7467462
GTACTTT67100.018.19581214
AGTACTT68200.017.84768313
TCGCGTA1200.001509796117.1942929
ACATGGG72300.017.0740383
Produced by FastQC (version 0.11.5)