FastQCFastQC Report
Mon 6 Feb 2017
SRR2077173_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077173_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262994
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.031.064251
GGTATCA13800.026.2260741
ATCAACG29950.024.0968933
TCAACGC29950.023.8636574
CAACGCA30950.022.980045
AACGCAG32150.021.9060756
TATCAAC33050.021.797542
GTACATG45800.020.3951471
TACATGG45150.020.1548162
GTATACG950.00787804418.1413441
ACATGGG47600.018.0635873
ACGCAGA38900.017.9273937
CGCAGAG39750.017.5455958
GAGTACT21050.016.54677412
GTATAGC7150.016.390641
AGAGTAC39000.015.91736611
GCTATAC6750.015.858773
GTGTAGC11850.015.4163151
GTCGTAG2705.3139956E-815.3193571
CAGAGTA43700.014.74839710
Produced by FastQC (version 0.11.5)