FastQCFastQC Report
Mon 6 Feb 2017
SRR2077173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262994
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.036.5115471
GTATCAA42300.033.3048321
TCAACGC54350.025.2191834
ATCAACG54600.025.1667843
TATCAAC55900.024.6431182
CAACGCA55600.024.5283225
CTATGCG1502.9438525E-822.9601889
AACGCAG59050.022.6286836
TCGATCG900.00554361919.28021241
ACGCAGA69300.018.9221447
CGCAGAG70000.018.5985748
TACGCTG1702.4760611E-618.2322855
AGAGTAC69550.017.87349511
TACATGG46400.017.664712
GAGTACT52000.017.61480112
GTACATG48550.017.523951
CAGAGTA74300.017.24217410
AGTACTT54150.016.9786313
GTACTTT55850.016.650114
GCAGAGT77900.016.3580289
Produced by FastQC (version 0.11.5)