FastQCFastQC Report
Mon 6 Feb 2017
SRR2077172_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077172_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232179
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51500.029.2491021
GGTATCA28250.025.5015261
TATCAAC62550.023.752822
ATCAACG61250.023.671613
TCAACGC61700.023.4964144
CAACGCA62200.023.307175
AACGCAG65350.022.233786
ACGCAGA75650.019.160667
GTACATG66600.019.0981141
TACATGG64550.018.958212
AGAGTAC69900.018.74822211
CGCAGAG76550.018.5770178
TAGCCCT17000.018.2738914
CGTATAC2501.0422809E-917.950973
GTGTAGC15750.017.7273081
ACATGGG69850.017.2481923
GTAGCCC13850.017.1982363
GTATACG1855.498745E-616.7690751
CAGAGTA82300.016.41663710
TATACTG11100.016.1700385
Produced by FastQC (version 0.11.5)