FastQCFastQC Report
Mon 6 Feb 2017
SRR2077172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232179
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.036.4069821
GTATCAA75000.034.7319451
TATCAAC97850.026.4062252
TCAACGC98100.026.1635724
ATCAACG98450.026.1404533
CAACGCA98950.025.798945
AACGCAG103050.024.6389476
ACGCAGA117300.021.3329267
CGCAGAG119250.020.7676338
AGAGTAC114550.020.2960111
GAGTACT93150.019.64209712
GTACATG61500.019.1357481
CGCGTGA900.00586437519.0937363
CAGAGTA124250.019.01689310
AGTACTT95400.018.99858913
CGTATAC1852.724173E-718.597543
TACATGG61550.018.3905642
GCAGAGT129050.018.3900879
GTGGTAT19450.018.0457551
GTACTTT101650.017.73718614
Produced by FastQC (version 0.11.5)