FastQCFastQC Report
Mon 6 Feb 2017
SRR2077171_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077171_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489879
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46500.032.5361861
GGTATCA23550.031.9022031
ATCAACG53850.027.3103433
TCAACGC54250.027.1074184
CAACGCA56800.025.9515955
TATCAAC59200.024.8027042
AACGCAG58900.024.730796
ACGCAGA68200.021.0041247
GTACATG75200.020.3479271
CGCAGAG71750.019.9179338
TACATGG74200.019.6957762
ACATGGG73850.018.1377943
AGAGTAC71200.018.11323211
GCAGAGT78050.017.5143649
CAGAGTA75200.017.50705110
GAGTACT42800.016.67725812
TGGACCG4051.8189894E-1215.3427075
CGGTTCG1350.003254269215.32663230
CATGGGG48550.015.14497954
TTGCGCG1400.00414787314.77776851
Produced by FastQC (version 0.11.5)