FastQCFastQC Report
Mon 6 Feb 2017
SRR2077171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489879
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.040.4095081
GTATCAA74900.035.6777951
ATCAACG98900.026.7019373
TCAACGC99150.026.4265274
TATCAAC101000.025.9424762
CAACGCA101200.025.4831055
AACGCAG104050.024.8511986
ACGCAGA119000.021.3369717
CGCAGAG120700.020.739088
AGAGTAC119300.019.91203911
CAGAGTA125150.019.42273110
GAGTACT95000.019.39659712
GCAGAGT129900.018.8456579
GTACATG70950.018.7108611
GTGGTAT16300.018.567521
AGTACTT101650.018.29650113
TGGTATC16300.018.1421472
TACATGG69000.017.9403652
GTACTTT105550.017.6924514
TACACCG2802.6739144E-1017.1926025
Produced by FastQC (version 0.11.5)