FastQCFastQC Report
Mon 6 Feb 2017
SRR2077170_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077170_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727414
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34450.035.4166071
GGTATCA17050.031.535021
TCAACGC42700.027.15984
ATCAACG43200.027.0866363
CAACGCA44250.025.9744345
TATCAAC45450.025.7875272
AACGCAG45750.025.1186586
ACGCAGA53300.020.718497
CGCAGAG54500.020.0115998
GTACATG54300.019.105551
AGAGTAC54900.018.84261511
GTATAGA7750.018.6784361
GAGTACT34950.017.85795212
TACATGG55850.017.5908322
CAGAGTA60100.017.08845710
GTGTAGC14750.016.824161
AGTACTT38550.016.54959913
GTACTTT36950.016.51988214
ACATGGG56750.016.2399443
GCAGAGT64800.016.1377479
Produced by FastQC (version 0.11.5)