FastQCFastQC Report
Mon 6 Feb 2017
SRR2077170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727414
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.038.719161
GTATCAA54100.036.7155951
CGCGTAA605.540575E-428.64581961
ATCAACG70150.027.2306253
TCAACGC70650.026.9891914
TATCAAC73550.026.486592
CAACGCA73050.026.0082515
AACGCAG76000.025.044016
ACGCAGA87550.021.4051487
CGCAGAG89300.020.8419728
AGAGTAC87850.019.81098711
GAGTACT67200.019.75674412
CAGAGTA93200.018.89727810
AGTACTT71700.018.51678313
GCAGAGT98650.018.027969
GTACTTT74100.018.0191414
GTACATG54800.017.3380621
GTGGTAT11800.017.2124231
TACATGG53200.017.0151772
ACATGGG54500.015.7251633
Produced by FastQC (version 0.11.5)