FastQCFastQC Report
Mon 6 Feb 2017
SRR2077169_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077169_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390132
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49900.034.461071
GGTATCA25550.033.044861
ATCAACG59350.027.74373
TCAACGC59100.027.7425964
CAACGCA61300.026.8595625
TATCAAC63650.026.046682
AACGCAG63700.025.7376866
TCGATCG953.0659267E-421.79094341
ACGCAGA75350.021.3915637
CGCAGAG77050.020.7410188
ATCGCGT1004.3235684E-420.7096618
GTACATG81150.019.4069121
AGAGTAC76200.019.0496411
TACATGG79300.019.0373542
CGATCGT1108.238326E-418.81917442
CAGAGTA80800.018.29786110
ACATGGG79900.017.3678473
GCAGAGT85800.017.2987929
GAGTACT42900.017.03876912
GTACCGT3301.546141E-1015.6889066
Produced by FastQC (version 0.11.5)