FastQCFastQC Report
Mon 6 Feb 2017
SRR2077169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390132
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35700.037.9287761
GTATCAA76550.035.781121
ATCAACG96200.027.5382773
TATCAAC97900.027.3408622
TCAACGC96450.027.2531454
CAACGCA99250.026.4492825
AACGCAG102050.025.6899096
ACGCAGA116900.022.0858767
CGCAGAG119450.021.4865268
AGAGTAC117600.020.30409611
GAGTACT85150.019.85119212
CAGAGTA123700.019.72003210
GCAGAGT129100.019.2683329
GTGGTAT16900.018.9119831
AGTACTT90850.018.6810713
GTACTTT93000.018.21951714
GTACATG79100.017.248641
TGGTATC17200.016.9803982
TACATGG78600.016.69932
AATACCG2001.1882272E-515.4619245
Produced by FastQC (version 0.11.5)