FastQCFastQC Report
Mon 6 Feb 2017
SRR2077168_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077168_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139386
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39150.031.515221
GGTATCA19350.030.8130151
TCAACGC44150.026.81414
ATCAACG44900.026.4464513
CAACGCA46650.025.3775235
TATCAAC48750.024.8150652
AACGCAG48750.024.4239866
ACGCAGA56700.020.877727
ATACCGT2001.2769306E-920.7072936
CGCAGAG57650.020.4162378
TACATGG57850.019.6604522
GTACATG61300.019.0593591
TATACCG1451.2612338E-519.0427075
AGAGTAC57700.019.00478211
ACATGGG60850.018.1528453
CAGAGTA64800.017.33795210
GAGTACT38550.016.99522412
GCAGAGT68550.016.5662029
TAGCCCT20250.016.3623624
AGTACTT41700.015.96050613
Produced by FastQC (version 0.11.5)