FastQCFastQC Report
Mon 6 Feb 2017
SRR2077168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139386
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.036.6141
GTATCAA57700.033.5657351
TCAACGC72800.025.4650654
ATCAACG73650.025.2645683
TATCAAC75650.024.869432
CAACGCA74800.024.6918225
AACGCAG77500.023.6096956
ACGCAGA90400.019.9200427
CGCAGAG91000.019.7276338
GTACATG59000.018.6578481
AGAGTAC91000.018.55251911
TACATGG57350.018.05182
CAGAGTA96950.017.6627410
GATCGTA1200.001511104317.19225111
GAGTACT73050.017.08634612
GTGGTAT15200.016.9738851
GCAGAGT101300.016.8377279
AGTACTT77150.016.4454713
GTACTTT78200.016.01042414
ACATGGG58850.015.9552493
Produced by FastQC (version 0.11.5)