FastQCFastQC Report
Mon 6 Feb 2017
SRR2077167_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077167_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319401
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.034.1350141
GTATCAA46450.030.2004221
TCAACGC57900.024.1469554
ATCAACG59450.023.7507343
TATCAAC59100.023.6803842
CAACGCA60500.023.1092385
AACGCAG64700.021.6601666
GTACATG72550.019.193241
ACGCAGA74000.018.6114947
TACATGG73700.018.47482
CGCAGAG74250.018.3166628
AGAGTAC73200.017.19318811
ACATGGG74850.017.1114863
GAGTACT44250.015.97405612
CAGAGTA79100.015.94689710
GCAGAGT83850.015.3553219
CATGGGG50400.014.9317914
CCTATAC8150.014.8257283
AGTACTT50150.014.30209913
GTACTTT50250.014.20565714
Produced by FastQC (version 0.11.5)