FastQCFastQC Report
Mon 6 Feb 2017
SRR2077167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319401
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32450.040.5887571
GTATCAA66500.032.5792121
ATCAACG81900.025.8194733
TCAACGC82550.025.4495644
TATCAAC83750.025.1670232
CAACGCA85800.024.3646055
AACGCAG88500.023.6212776
ACGCAGA102750.019.9959327
CGCAGAG104750.019.196438
GTACATG67950.019.0797881
CGATCGT1501.6141807E-518.50529142
AGAGTAC101600.018.50375611
TCGATCG1552.0776928E-517.97042741
GAGTACT76150.017.60195712
CAGAGTA109050.017.55585710
TACATGG68500.017.4680672
GTGGTAT16650.017.142951
GCAGAGT112450.017.1177719
AGTACTT81400.016.80447413
GTACTTT82150.016.70019514
Produced by FastQC (version 0.11.5)