FastQCFastQC Report
Mon 6 Feb 2017
SRR2077166_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077166_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656685
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.031.1738721
GTATCAA33150.030.7746431
TCAACGC37950.025.3794214
ATCAACG39000.025.0520883
CAACGCA39600.024.4086635
TATCAAC40900.024.1001322
AACGCAG41750.023.2330576
ACGCAGA49950.019.2124257
GTACATG61950.018.91571
CGCAGAG51300.018.5729568
TACATGG60300.018.1755032
AGAGTAC50300.017.20466211
ACATGGG61750.016.9405613
CAGAGTA56700.015.74173110
GCAGAGT58550.015.5066569
CATGGGG42200.015.4610394
GTGTAGC11850.015.4149591
GTATAGC8750.015.3617751
ACGCCCG2252.04977E-615.33361421
TTAGTCG1605.785576E-415.08548939
Produced by FastQC (version 0.11.5)