FastQCFastQC Report
Mon 6 Feb 2017
SRR2077166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656685
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.041.0888941
GTATCAA56950.035.3518941
TATCAAC74300.026.721692
ATCAACG75500.026.3881243
TCAACGC75050.026.2712554
CAACGCA76500.025.8173085
AACGCAG79450.024.6854746
ACGCAGA90550.021.3001757
CGCAGAG92450.020.6877778
GAGTACT67100.019.58038912
AGAGTAC91500.019.39586411
AGTACTT70350.019.01805113
CAGAGTA96550.018.70303510
GTACATG62450.018.5164361
GTACTTT73900.018.38994614
GCAGAGT100900.018.1704489
TACATGG60750.018.0684662
TACACCG3257.2759576E-1216.9393655
ACATGGG62400.016.8738333
GTGGTAT12700.016.801131
Produced by FastQC (version 0.11.5)