FastQCFastQC Report
Mon 6 Feb 2017
SRR2077165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395044
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35600.031.1759191
GGTATCA18950.027.101321
ATCAACG39550.026.8876973
TCAACGC39600.026.8520624
CAACGCA41100.026.0406065
TATCAAC43250.024.8635832
AACGCAG43600.024.4662176
GTACATG58150.020.6865981
ACGCAGA50700.020.4948277
TACATGG57600.019.6865042
CGCAGAG52200.019.3123198
ACATGGG59200.018.0796243
AGAGTAC50550.017.86759811
CGCACGA1200.001478168217.24867642
TGTACCG2702.8976501E-916.6230555
CAGAGTA56300.016.46724510
GAGTACT28150.016.2876812
GCAGAGT59100.016.1823549
CATGGGG37400.015.9696944
GTATAGC8100.015.7445441
Produced by FastQC (version 0.11.5)