FastQCFastQC Report
Mon 6 Feb 2017
SRR2077165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395044
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.037.9083561
GTATCAA53700.035.4118421
ATCAACG67550.027.8417533
TCAACGC67700.027.6783054
TATCAAC69350.027.3674552
CAACGCA68800.027.134515
AACGCAG72800.025.6909166
ACGCAGA83100.022.2355987
CGCAGAG84300.021.4821958
GAGTACT60100.020.62025612
AGAGTAC82050.020.43225911
AGTACTT63600.019.59374613
CAGAGTA88350.019.4432610
GTACTTT65450.019.30814214
GCAGAGT93100.018.5999419
GTACATG58900.018.5992571
TACATGG57000.018.5520882
GTGGTAT12200.018.3542771
ACCGTGC3103.6379788E-1217.7696468
ACATGGG56700.017.7389983
Produced by FastQC (version 0.11.5)