FastQCFastQC Report
Mon 6 Feb 2017
SRR2077164_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077164_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970691
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.032.7037471
GTATCAA40050.031.8423421
TCAACGC51300.024.017514
ATCAACG51200.023.9994433
CAACGCA52250.023.513985
TATCAAC54350.022.831692
AACGCAG55650.021.8887376
GTACATG76900.020.3486371
TACATGG76700.019.594142
ACGCAGA65850.018.1317127
ACATGGG79300.018.0632553
CGCAGAG67350.017.5750668
AGAGTAC67450.016.1558811
ACCGTGC4700.016.153458
GTATATA13750.015.7922371
GTACGAC1554.511137E-415.5878943
CAGAGTA71650.015.58284710
CATGGGG55950.015.1744224
GCAGAGT76300.014.8798039
GTATAGG8600.014.8289051
Produced by FastQC (version 0.11.5)