FastQCFastQC Report
Mon 6 Feb 2017
SRR2077164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970691
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.041.7910421
GTATCAA61650.034.7059171
TATCAAC80800.025.8387992
ATCAACG79900.025.7424223
TCAACGC80550.025.5346954
CAACGCA81800.025.1015995
AACGCAG84600.023.9455786
GTACATG72000.020.5438671
ACGCAGA98550.020.1212867
CGCAGAG100150.019.5704968
TACATGG73250.019.1578852
AGAGTAC98050.018.62068211
GAGTACT71600.018.15212412
ATGCGCG1150.001120858217.975851
AGTACTT73950.017.85395213
CAGAGTA104500.017.83359710
ACATGGG77100.017.7105123
GTACTTT76350.017.43546314
GCAGAGT108800.017.4134989
GTGGTAT14800.016.9670891
Produced by FastQC (version 0.11.5)