FastQCFastQC Report
Mon 6 Feb 2017
SRR2077163_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077163_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734926
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42600.029.2038351
GGTATCA24250.025.864371
ATCAACG49350.023.8546683
TCAACGC49500.023.6411654
TATCAAC53750.022.634472
CAACGCA52500.022.4209215
AACGCAG54550.021.259996
ACGCAGA62300.018.228147
GTACATG58700.017.9649311
AGAGTAC58850.017.69235611
CGCAGAG63150.017.6558078
TACATGG56950.017.0053922
GAGTACT37300.016.63783512
GTATAGC9550.016.2387071
ACATGGG58250.015.9427253
GTGTAGC15400.015.664611
CAGAGTA68450.015.65831910
GCAGAGT70750.015.2239169
TATACTG12650.015.0082685
TATACCG1851.0173433E-414.9271425
Produced by FastQC (version 0.11.5)