FastQCFastQC Report
Mon 6 Feb 2017
SRR2077163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734926
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33450.035.1682051
GTATCAA67250.033.0415531
TCAACGC85950.025.0891674
ATCAACG86850.024.9875763
TATCAAC87550.024.9449232
CAACGCA88200.024.1757375
AACGCAG91600.023.1282026
ACGCAGA104400.019.8154057
GTACATG57850.019.5619771
AGAGTAC97900.019.42385911
CGCAGAG104650.019.2910338
GAGTACT75950.018.8350412
TACATGG57250.017.962232
AGTACTT80600.017.96172313
CAGAGTA110000.017.53860310
GTACTTT84200.017.48668914
GCAGAGT114300.017.1198069
ACATGGG57850.017.0030673
ACTTTTT100250.015.60359616
TACTTTT97100.015.57470715
Produced by FastQC (version 0.11.5)