FastQCFastQC Report
Mon 6 Feb 2017
SRR2077162_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077162_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162984
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.031.0323771
GTATCAA30750.030.8243791
TCAACGC33850.026.735934
ATCAACG33950.026.6596553
CAACGCA34900.025.9315535
TATCAAC37300.025.2278772
AACGCAG36750.024.8124166
ACGCAGA42950.020.747657
CGCAGAG42500.020.5624188
GTATAGC9100.018.5592941
AGAGTAC44100.017.59633411
ATAGCCC8250.017.1681273
TAGCCCT15900.017.1625824
CAGAGTA46250.016.98946210
GTACATG41050.016.9607471
GAGTACT26900.016.79644612
GCAGAGT49200.016.4989179
TACATGG40100.016.3318772
TATAGCC9250.016.0233612
GCCGATC1605.7084963E-415.1119568
Produced by FastQC (version 0.11.5)