FastQCFastQC Report
Mon 6 Feb 2017
SRR2077162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162984
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.041.7538071
GTATCAA48400.036.379771
CGCGAAG553.3198585E-431.26314218
ATCAACG58550.029.3641973
TCAACGC58750.028.7960054
TATCAAC62200.027.6963392
CAACGCA61800.027.3185715
AACGCAG63350.026.3787776
ACGCAGA74500.022.136987
CGCAGAG74100.021.999138
AGAGTAC75400.020.3845511
GAGTACT60900.020.10007512
CAGAGTA79150.019.7667610
CGTGCGC4100.019.28941210
GCAGAGT82200.019.1597029
GCACCGT2255.7752914E-918.3384956
GTACTTT68000.018.26182714
AGTACTT67800.018.2573513
GCGTTAA950.00802887418.08171768
GTACATG39750.017.9088841
Produced by FastQC (version 0.11.5)