FastQCFastQC Report
Mon 6 Feb 2017
SRR2077161_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077161_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643995
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39550.033.545941
GGTATCA19900.032.9022981
ATCAACG45600.027.479983
TCAACGC46050.027.0599784
CAACGCA47000.026.8067955
AACGCAG48900.025.693176
TATCAAC49400.025.6034052
ACGCAGA57150.021.6218057
CGCAGAG59050.020.8684398
GAGTACT38950.019.03355612
AGAGTAC59000.018.87656411
CAGAGTA63250.018.3057710
ATACCGT2105.0169547E-818.0800066
GCAGAGT66100.017.598619
GTACTTT42700.017.52482414
AGTACTT44000.017.16379213
TAGCCCT19000.016.7141424
GTGTAGC18250.016.6167281
GTACATG48350.016.036581
CGACTCG1300.002518718815.9270165
Produced by FastQC (version 0.11.5)