FastQCFastQC Report
Mon 6 Feb 2017
SRR2077161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643995
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.038.026631
GTATCAA53550.036.8327331
TCAACGC67450.028.0148494
ATCAACG68800.027.9645043
TATCAAC70750.027.5336722
CAACGCA68750.027.4351395
AACGCAG72050.026.1308866
ACGCAGA84100.021.96297
CGCAGAG85200.021.5715128
AGAGTAC84950.020.05368811
GAGTACT68100.020.0229312
CAGAGTA91450.019.23070110
TGGTATC10900.019.2269732
GCAGAGT93450.018.7841539
GTACTTT73950.018.72756614
GTACATG48050.018.5935151
AGTACTT73950.018.57830213
GTGGTAT12550.018.3447841
CTCGCGA950.00806028218.0696568
TACATGG48700.017.848392
Produced by FastQC (version 0.11.5)