FastQCFastQC Report
Mon 6 Feb 2017
SRR2077160_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077160_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943869
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.034.008921
GTATCAA47550.033.4878961
ATCAACG57300.027.4797273
TCAACGC57450.026.922554
CAACGCA59850.025.8420665
TATCAAC61450.025.465522
AACGCAG62750.024.59156
TATACGA1752.4374458E-1023.6353362
ACGCAGA70900.021.5702957
CGCAGAG71300.020.917048
GTACATG70100.019.9620171
AGAGTAC73750.019.0295411
TACATGG70950.018.509352
GAGTACT47450.018.385712
CAGAGTA78350.017.99021710
TATACCG1954.5665183E-717.7043025
GCAGAGT81400.017.602199
ACATGGG72800.017.3126543
AGTACTT51900.017.0776513
GTGTAGC15950.016.855061
Produced by FastQC (version 0.11.5)