FastQCFastQC Report
Mon 6 Feb 2017
SRR2077160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943869
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.041.6796151
GTATCAA69550.037.9429441
ATCAACG95150.026.8638653
TATCAAC96950.026.8618872
TCAACGC95450.026.5631774
CAACGCA97550.025.9560745
AACGCAG101450.024.8226136
ACGCAGA118450.021.1506217
CGCAGAG118550.020.7392738
AGAGTAC117100.019.78840811
GTACATG71300.019.4950621
GAGTACT91200.019.37653512
CAGAGTA122950.019.01658410
AGCGTAC2204.3746695E-918.75472510
AGTACTT95950.018.5961413
GCAGAGT128350.018.4314489
GTACTTT98750.018.0407914
TACATGG71600.018.0180052
ACATGGG72500.017.2249163
TACTTTT108850.016.6082915
Produced by FastQC (version 0.11.5)