FastQCFastQC Report
Mon 6 Feb 2017
SRR2077159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854988
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.037.0550421
GTATCAA51950.034.768391
TCAACGC62700.028.0229574
ATCAACG65050.027.3854273
CAACGCA65450.027.0032735
AACGCAG67600.025.8859446
TATCAAC68850.025.5828062
ACGCAGA78100.022.1847937
CGCAGAG78900.021.699298
AGAGTAC79250.020.1003411
GTACATG94300.019.8119131
TACATGG90900.019.6804352
GAGTACT37450.019.42601412
CAGAGTA84100.019.2190510
GCAGAGT87900.018.6145789
ACATGGG95300.018.2580643
AGTACTT41750.017.34388413
GTGTACG3850.017.0110741
GTACTTT41650.016.80659914
CATGGGG57650.015.6280554
Produced by FastQC (version 0.11.5)