FastQCFastQC Report
Mon 6 Feb 2017
SRR2077159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854988
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.1060950168617171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35100.046.8621371
GTATCAA83550.038.5917471
ATCAACG113300.028.3304843
TATCAAC113550.028.2686062
TCAACGC113050.028.1801094
CAACGCA115600.027.3501665
AACGCAG117900.026.5248176
ACGCAGA136850.022.5308137
CGCAGAG137550.022.0282758
GAGTACT95450.021.8316112
AGAGTAC134150.021.01900911
AGTACTT99700.020.86648413
GTGGTAT13250.020.2572291
CAGAGTA141350.020.21737510
GCAGAGT143550.020.0040539
GTACTTT105200.019.85171714
GTACATG91450.019.3034521
TGGTATC13450.018.9286352
TACATGG88400.018.836622
TACTTTT113550.018.50142115
Produced by FastQC (version 0.11.5)