FastQCFastQC Report
Mon 6 Feb 2017
SRR2077158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832329
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39950.032.6127851
GGTATCA20650.032.2144051
TATCGCG500.00765554727.65977562
ATCAACG48950.026.2344283
TCAACGC48550.026.233084
CAACGCA50000.025.7484465
TATCAAC52900.024.6938442
AACGCAG52500.024.3895456
ACGCAGA61050.021.313017
CGCAGAG62750.020.5170528
GTACATG68150.019.370721
GAGTACT31450.018.85740512
TACATGG66650.018.668622
AGAGTAC61100.018.45358311
CAGAGTA64000.018.30775310
GCAGAGT69100.017.5339479
CTATACG1200.001473106917.257674
ACATGGG70250.017.1989963
GTGGTAT12800.016.9645921
AGTACTT35800.016.75929513
Produced by FastQC (version 0.11.5)