FastQCFastQC Report
Mon 6 Feb 2017
SRR2077158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832329
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.041.17461
GTATCAA62800.034.5855261
TATCAAC83950.025.7419092
TCAACGC83300.025.6122954
ATCAACG84100.025.5732443
CAACGCA85450.024.8868855
AACGCAG87950.024.3750956
ACGCAGA100550.020.9267987
GTGGTAT14550.020.3451141
CGCAGAG103600.020.1250938
GAGTACT80250.019.41717712
AGAGTAC102250.019.37724711
GTACATG65000.019.1169451
AGTACTT84050.018.70300513
CAGAGTA106800.018.5855710
TCGATCG1501.5907348E-518.5365741
TACATGG63550.018.464632
TGGTATC14650.018.3214042
GCAGAGT111500.018.1418449
GTACTTT86750.018.08731714
Produced by FastQC (version 0.11.5)