FastQCFastQC Report
Mon 6 Feb 2017
SRR2077157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574998
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27400.032.5812071
GGTATCA15050.028.3990081
ATCAACG31350.026.0978573
TCAACGC31300.025.694374
TATCAAC34450.024.4123842
CAACGCA33500.024.3156095
AACGCAG35500.022.9430356
GTACATG54200.020.7317661
TACATGG51900.019.7241652
TACACCG2303.43789E-1019.508935
ACATGGG54150.019.1256773
ACGCAGA43000.019.0219737
CGCAGAG43050.018.5203138
AGAGTAC43200.018.03826311
ACCGCGC1602.94155E-517.2575658
GACCGCG1602.9435174E-517.256227
GTATAAG7750.016.9005131
CATGGGG37250.016.4937764
CAGAGTA47450.016.26873610
GTAGCCC11750.015.8653613
Produced by FastQC (version 0.11.5)