FastQCFastQC Report
Mon 6 Feb 2017
SRR2077157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574998
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45300.037.4843181
GGTATCA21700.036.8238451
ATCAACG59950.027.5720143
TCAACGC59750.027.3761374
TATCAAC61350.027.279612
CAACGCA61400.026.583345
AACGCAG63950.025.3617926
ACGCAGA74750.021.35847
CGCAGAG75150.021.115158
CGCGTAA850.004179972720.24483710
GAGTACT57950.019.65601712
GTGGTAT9650.019.6307221
AGAGTAC76850.019.47898711
TGGTATC10050.019.1883852
AGTACTT61500.019.13691713
GTACTTT60350.019.05280914
CAGAGTA81500.018.62276610
TCGTCGG1301.2036226E-418.5325329
GTACATG52450.017.99311
GCAGAGT85300.017.9552339
Produced by FastQC (version 0.11.5)