FastQCFastQC Report
Mon 6 Feb 2017
SRR2077156_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077156_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724466
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.032.8689421
GTATCAA39600.031.9438021
ATCAACG46400.026.2677753
TCAACGC46850.026.0121174
CAACGCA48500.025.1983495
AACGCAG50150.024.2982166
TATCAAC51250.024.0797942
ACGCAGA57150.021.0804487
CGCAGAG57950.021.0288668
GTACATG54800.018.806431
GTATAGC10550.018.2957951
AGAGTAC59700.018.02102711
ACTGCGT3103.6379788E-1217.817758
GAGTACT40950.017.51494412
TACATGG54650.017.4724252
CAGAGTA65750.017.40276110
GCAGAGT66450.017.299919
ACATGGG56050.016.8171943
ATACCGT2301.4154648E-716.5095586
GTATTAC5900.016.3576811
Produced by FastQC (version 0.11.5)