FastQCFastQC Report
Mon 6 Feb 2017
SRR2077156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724466
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.038.6857951
GTATCAA61350.037.2896421
ATCAACG80050.028.101773
TCAACGC80450.027.6200054
TATCAAC82300.027.5842632
CAACGCA82550.026.7918825
AACGCAG85450.025.6411026
ACGCAGA97900.022.1866997
CGCAGAG97950.022.0875518
AGAGTAC98150.021.12868511
GAGTACT80150.020.7251212
CAGAGTA102650.020.67262510
GTACATG50850.019.8901251
GCAGAGT107350.019.7034599
AGTACTT86700.019.3577213
GTACTTT88500.018.89530814
TACATGG51900.018.5570092
GTGGTAT12500.018.164151
CCGCGAT1150.001134157617.9441479
ACATGGG52650.017.5740223
Produced by FastQC (version 0.11.5)