FastQCFastQC Report
Mon 6 Feb 2017
SRR2077155_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077155_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865657
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26200.031.173361
GGTATCA14000.030.0309031
TCAACGC29050.026.8555344
CAACGCA30100.026.4921345
ATCAACG29250.026.0832233
AACGCAG32200.024.656526
TATCAAC31500.024.6174762
ACGCAGA37350.021.1649047
CCCTACG1356.8754216E-620.42777312
CGCAGAG38200.020.242678
GTATAAT6500.018.5562931
AGAGTAC36800.018.4536911
GTACATG34300.017.281081
CAGAGTA41100.017.10553210
TACATGG34500.016.68282
GAGTACT24250.016.63178612
GCAGAGT43150.016.4000269
GTGGTAT9250.016.020031
ACATGGG36300.015.9770823
GTATAGC6500.015.9053951
Produced by FastQC (version 0.11.5)