FastQCFastQC Report
Mon 6 Feb 2017
SRR2077155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865657
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.039.2977941
GTATCAA39100.033.2423171
ATCAACG50300.025.0810723
TATCAAC52050.024.5680242
TCAACGC50700.024.4085834
CAACGCA51550.023.8054225
GTGGTAT9350.022.8011261
TGGTATC8900.022.7881982
AACGCAG52950.022.7215756
TAGACCG953.1430938E-421.7102075
CGGCCTA800.00292217221.53433851
GTACATG36300.019.5135751
ACGCAGA61100.019.5026477
CGCAGAG62900.018.8459648
AGAGTAC61500.018.77114511
CGACCCG950.00788936618.1366239
GAGTACT50350.017.94662912
ACTGCGT3153.6379788E-1217.4525668
TACATGG37450.017.4401342
CAGAGTA66800.017.43705610
Produced by FastQC (version 0.11.5)