FastQCFastQC Report
Mon 6 Feb 2017
SRR2077154_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077154_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801686
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.036.6447681
GTATCAA30250.032.1279871
ATCAACG38350.024.5845933
TCAACGC38100.024.56124
TATCAAC38650.024.5260412
CAACGCA39200.023.9600735
AACGCAG40550.023.2449556
TATACGG1554.051435E-822.2778665
GTACATG51200.021.8089541
TACATGG49850.021.574152
ACATGGG51050.020.2274533
ACGCAGA46950.019.929797
CGCAGAG46450.019.9229838
CGTACAC2204.138201E-918.8375433
AGAGTAC45050.018.21170211
GAATACG1903.510504E-718.15282223
GTTACGA1150.001102298418.02002769
GTCTTAG4800.017.9497571
GGGCGAC1351.5873443E-417.87444911
CATGGGG31400.017.7052274
Produced by FastQC (version 0.11.5)