FastQCFastQC Report
Mon 6 Feb 2017
SRR2077154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801686
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.10973055238260163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.044.1881561
GTATCAA45750.039.2582551
TATCAAC64200.027.4371012
ATCAACG64900.027.141173
TCAACGC65150.026.772994
CAACGCA66400.026.2157125
AACGCAG69000.025.1281556
ACGCAGA80650.021.0168887
TAACGTC1004.1532534E-420.83257544
CGCAGAG81400.020.5806758
GTGGTAT7850.020.1622871
AGAGTAC78350.019.92964211
CAGAGTA82250.019.23715210
GAGTACT61800.019.03357112
GCAGAGT85950.018.7307439
GTACATG48000.018.2789211
AGTACTT65050.018.1883713
GTGTAGC8950.018.068681
TACATGG48250.017.7543722
CGCCCGG1753.3159959E-617.68244723
Produced by FastQC (version 0.11.5)