FastQCFastQC Report
Mon 6 Feb 2017
SRR2077153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917869
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38100.024.206891
GGTATCA18100.022.6195681
CGCAATA809.008214E-519.24550436
GTGTACG1404.0599843E-918.867011
TATCAAC51250.017.95142
TCAACGC52200.017.2869324
ATCAACG52600.017.2396093
GTCCTAA2201.8189894E-1217.0088981
CAACGCA53100.016.9088275
AGCGTAC953.3342748E-416.219011
AACGCAG55600.016.1417986
GTATTAA2600.016.0853391
CGCGTAA1105.636507E-515.99584510
TACGGGA850.002971967215.5363232
GTTATCG1158.2563E-515.29678243
ATACCGT2051.8153514E-915.0224486
GTATAGA2509.094947E-1214.9678291
GTACATG92000.014.7859951
GTACGGG900.00434511114.6743421
CTAGCAC2102.5302143E-914.6731933
Produced by FastQC (version 0.11.5)