FastQCFastQC Report
Mon 6 Feb 2017
SRR2077153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917869
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22350.11653559236840473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAA300.005740252329.33917610
GGTATCA21350.026.2774681
GTATCAA41550.022.2921681
ATACCGT1653.6379788E-1220.0013756
TAATACC1351.0845924E-616.298694
TATAGGG1501.8361425E-716.1357062
TATCAAC57700.016.0543542
CGCGTAA1105.614426E-516.00318710
GTATATA1401.5866062E-615.7149551
ATCAACG59050.015.6127963
TCAACGC58750.015.542714
CAACGCA59900.015.1341115
ACCGTCG1756.3993866E-815.088728
TTAATAC2203.0377123E-1015.0022063
AACGCAG61500.014.7392246
GTCTTAC2254.274625E-1014.6672931
TAGGACA4100.014.4899354
CTAGACA3050.014.4283514
GGACCGT1851.265671E-714.2712516
GTACATG76700.014.0553591
Produced by FastQC (version 0.11.5)