##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077153_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1917869 Sequences flagged as poor quality 0 Sequence length 50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.30836464847182 31.0 31.0 34.0 27.0 34.0 2 30.409848639297053 31.0 31.0 34.0 27.0 34.0 3 30.48462381945795 31.0 31.0 34.0 27.0 34.0 4 34.00933431845449 37.0 35.0 37.0 30.0 37.0 5 33.5014768996214 37.0 35.0 37.0 28.0 37.0 6 33.41876217823011 37.0 35.0 37.0 28.0 37.0 7 33.227516582206604 37.0 35.0 37.0 26.0 37.0 8 33.143009767611865 37.0 35.0 37.0 26.0 37.0 9 34.54408408499225 39.0 35.0 39.0 27.0 39.0 10 34.39998352337933 39.0 35.0 39.0 26.0 39.0 11 34.39854599036743 39.0 35.0 39.0 26.0 39.0 12 34.30416415302609 39.0 35.0 39.0 26.0 39.0 13 34.26859498745743 39.0 35.0 39.0 25.0 39.0 14 35.29567660773494 39.0 35.0 41.0 25.0 41.0 15 35.28569625975497 39.0 35.0 41.0 25.0 41.0 16 35.2511824321682 39.0 35.0 41.0 25.0 41.0 17 35.232654055099694 39.0 35.0 41.0 25.0 41.0 18 35.14585719879721 39.0 35.0 41.0 25.0 41.0 19 35.18989722447154 39.0 35.0 41.0 25.0 41.0 20 35.162414116918306 39.0 35.0 41.0 25.0 41.0 21 35.120004025301 39.0 35.0 41.0 25.0 41.0 22 35.02171055478763 39.0 35.0 41.0 24.0 41.0 23 34.96341355952883 39.0 34.0 41.0 24.0 41.0 24 34.939623613500196 39.0 34.0 41.0 24.0 41.0 25 34.86560083092224 39.0 34.0 41.0 23.0 41.0 26 34.78993716463429 39.0 34.0 41.0 23.0 41.0 27 34.66854096916943 39.0 34.0 41.0 22.0 41.0 28 34.62174475941787 39.0 34.0 41.0 22.0 41.0 29 34.57212875331944 39.0 34.0 41.0 21.0 41.0 30 34.48438866262503 39.0 34.0 41.0 21.0 41.0 31 34.38056457453559 39.0 34.0 41.0 19.0 41.0 32 34.29077898438319 39.0 34.0 41.0 19.0 41.0 33 34.1661432558741 39.0 34.0 41.0 18.0 41.0 34 34.09810628358871 39.0 33.0 41.0 18.0 41.0 35 34.017756687239846 38.0 33.0 41.0 16.0 41.0 36 33.92231690485638 38.0 33.0 41.0 16.0 41.0 37 33.81867478957113 38.0 33.0 41.0 15.0 41.0 38 33.63740849870351 38.0 33.0 40.0 14.0 41.0 39 33.47729537314592 38.0 33.0 40.0 9.0 41.0 40 33.42570738668804 38.0 33.0 40.0 9.0 41.0 41 33.28928044616186 38.0 33.0 40.0 9.0 41.0 42 33.41458671056261 38.0 33.0 40.0 8.0 41.0 43 33.39023363952387 38.0 33.0 40.0 7.0 41.0 44 33.285809927581084 38.0 33.0 40.0 2.0 41.0 45 33.116130976620404 38.0 33.0 40.0 2.0 41.0 46 32.97141775585298 38.0 33.0 40.0 2.0 41.0 47 32.80249798083185 38.0 33.0 40.0 2.0 41.0 48 32.6229758132594 38.0 32.0 40.0 2.0 41.0 49 32.34774846457188 38.0 32.0 40.0 2.0 41.0 50 32.19497056368292 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7723883107766305 1101 2 0.9991855005781964 1101 3 1.0416263982696599 1101 4 1.128695861410229 1101 5 1.4124188383341902 1101 6 1.4353151149044336 1101 7 1.4428341366460913 1101 8 1.4432725050236428 1101 9 1.754114911766031 1101 10 1.8586312412163366 1101 11 1.8083170552715586 1101 12 1.7505259760428373 1101 13 1.8973143474077574 1101 14 2.074228218333907 1101 15 2.0083958399885233 1101 16 2.0880520913782235 1101 17 2.054193950425052 1101 18 2.0104575926934984 1101 19 2.093894930971601 1101 20 1.9864523453187033 1101 21 2.0204376054594064 1101 22 2.1270629048534673 1101 23 2.195003422347611 1101 24 2.0976674963272686 1101 25 2.0692175543017157 1101 26 2.20672427328838 1101 27 2.300697145988636 1101 28 2.2729157663078894 1101 29 2.3170827750061704 1101 30 2.150899278566868 1101 31 2.185358936459984 1101 32 2.3454455247958137 1101 33 2.3604934677415272 1101 34 2.354202330666034 1101 35 2.36816956720768 1101 36 2.4142492865675536 1101 37 2.3439638399109555 1101 38 2.363100732921012 1101 39 2.7008242389153736 1101 40 2.5242978559289924 1101 41 2.543323023048366 1101 42 2.44477241057605 1101 43 2.450556693684902 1101 44 2.413956392494434 1101 45 2.4974784028887314 1101 46 2.4608407714538743 1101 47 2.53832478902131 1101 48 2.4670059702988993 1101 49 2.7873426290292045 1101 50 2.788030779334683 1102 1 0.5033690107703386 1102 2 0.5950937579248645 1102 3 0.607394473601623 1102 4 0.6470750848391802 1102 5 0.7049031966180905 1102 6 0.8524667382534545 1102 7 0.9198222934353169 1102 8 0.9658200926056608 1102 9 1.0337922182222172 1102 10 1.0903621473925398 1102 11 0.8888193125144426 1102 12 0.9489756603379718 1102 13 1.0932213902926122 1102 14 1.1533040349766708 1102 15 1.180289660696424 1102 16 1.2553712552543885 1102 17 1.1966746101207093 1102 18 1.2079721413939666 1102 19 1.0813870783719324 1102 20 1.0918483095517004 1102 21 1.19858645652333 1102 22 1.3282385038867517 1102 23 1.2766472736930652 1102 24 1.2438279753110066 1102 25 1.214410328033125 1102 26 1.2006988866203017 1102 27 1.3100115160528105 1102 28 1.366917318610895 1102 29 1.3006903006947965 1102 30 1.2842891395772327 1102 31 1.3871268616403043 1102 32 1.3278568056003621 1102 33 1.3808605443442588 1102 34 1.4595281873308323 1102 35 1.537960712897835 1102 36 1.411723758028252 1102 37 1.397865519290832 1102 38 1.6239146873080301 1102 39 1.562629525357238 1102 40 1.464131761715457 1102 41 1.5597309541464242 1102 42 1.5968092286419449 1102 43 1.4700151320415742 1102 44 1.3521678877066918 1102 45 1.5132967679802434 1102 46 1.4979210506917653 1102 47 1.509470280442244 1102 48 1.609091377877803 1102 49 1.7280653000502681 1102 50 1.7076251270086047 1103 1 0.2731952227984955 1103 2 0.36571461009796025 1103 3 0.3791673619788476 1103 4 0.25234894768014016 1103 5 0.44055736647271715 1103 6 0.4121394328502461 1103 7 0.49664999887316696 1103 8 0.4916535113485736 1103 9 0.6102980156126847 1103 10 0.5854297110054603 1103 11 0.6905730823873952 1103 12 0.5911754615521403 1103 13 0.48126916033453426 1103 14 0.7069393977740575 1103 15 0.6368431974078419 1103 16 0.6782599105580047 1103 17 0.640729083689159 1103 18 0.666268033550665 1103 19 0.6727610285292869 1103 20 0.6978498090526131 1103 21 0.6499211368120967 1103 22 0.851609213511928 1103 23 0.8345160384957353 1103 24 0.7764985302984897 1103 25 0.8983567432539843 1103 26 0.7738575553749314 1103 27 0.9248154990789459 1103 28 0.7549816408971282 1103 29 0.6684536263770227 1103 30 0.7165198815706475 1103 31 0.6407674946722821 1103 32 0.6863332628798133 1103 33 0.7125161421941115 1103 34 0.8143399777195626 1103 35 0.7517292404869806 1103 36 0.7792027823621694 1103 37 0.6525323914701602 1103 38 0.6429710875577186 1103 39 0.7255036093891363 1103 40 0.8122935224293286 1103 41 0.8627095900984827 1103 42 0.8238277615940177 1103 43 0.8034357288069245 1103 44 0.850376670036205 1103 45 0.9247147179755189 1103 46 0.6824089229285661 1103 47 0.6794467338815693 1103 48 0.6401526574642773 1103 49 0.600968428636314 1103 50 0.6738133940892581 1104 1 0.2960171008070134 1104 2 0.3243542571584861 1104 3 0.2618246890444915 1104 4 0.24610396688990477 1104 5 0.3600404678643656 1104 6 0.1722671401268414 1104 7 0.19534361082554597 1104 8 0.19533588233553445 1104 9 0.2817700487651038 1104 10 0.3418014838299186 1104 11 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1104 48 0.5711172334558867 1104 49 0.5743923365560448 1104 50 0.5935067259637137 1105 1 0.40584483652039083 1105 2 0.4446301637755212 1105 3 0.4668877503927149 1105 4 0.2979487051976477 1105 5 0.4601717281748421 1105 6 0.5461072114013206 1105 7 0.5480432375333848 1105 8 0.5646961832957302 1105 9 0.8183654145138703 1105 10 0.6776923884024839 1105 11 0.798717833571267 1105 12 0.7275905043041746 1105 13 0.6800922595074752 1105 14 0.6393537325950547 1105 15 0.7675023879913496 1105 16 0.7643712288010818 1105 17 0.752738572174465 1105 18 0.7936317545245757 1105 19 0.8601598790849536 1105 20 0.800940310072626 1105 21 0.752387134948826 1105 22 0.7906056269068245 1105 23 0.8704082111167608 1105 24 0.912172731045267 1105 25 0.9547632659777463 1105 26 0.9240499809731304 1105 27 0.9416670655489767 1105 28 0.8441941849932206 1105 29 0.84276656963943 1105 30 0.7970775314605447 1105 31 0.659770126629148 1105 32 0.7481028925462638 1105 33 0.8042288842163074 1105 34 0.8706817141666932 1105 35 0.8516254213417298 1105 36 0.7832105557947813 1105 37 0.7167710341942168 1105 38 0.875710205304415 1105 39 0.9870964404136444 1105 40 0.9458250580413008 1105 41 0.970710499637967 1105 42 0.8175460243735984 1105 43 0.8112993788956686 1105 44 0.841170689722027 1105 45 0.8722040023483757 1105 46 0.976846670106049 1105 47 0.893564112345743 1105 48 0.9278002735134123 1105 49 0.8813123413499824 1105 50 1.0047410046885261 1106 1 0.2493766818826515 1106 2 0.2169301742052241 1106 3 0.2589211949762209 1106 4 0.17244851398641003 1106 5 0.26572076516552556 1106 6 0.20777500795256287 1106 7 0.3036226944934697 1106 8 0.3273782685411746 1106 9 0.27028926029331757 1106 10 0.36986967598418374 1106 11 0.2919220124059265 1106 12 0.1600625179323245 1106 13 0.1720618221552357 1106 14 0.3177898854249861 1106 15 0.37447681783420705 1106 16 0.35968438065756914 1106 17 0.2528867608825749 1106 18 0.4088830234718728 1106 19 0.38575046106242183 1106 20 0.3974446210168594 1106 21 0.3191176835512479 1106 22 0.3725344267955748 1106 23 0.3861714046920639 1106 24 0.3197029771862816 1106 25 0.2811647574253868 1106 26 0.3054255719896801 1106 27 0.4751942175445265 1106 28 0.4974764996921479 1106 29 0.4233555871613319 1106 30 0.3992525592730871 1106 31 0.38655577883329784 1106 32 0.3161441072590989 1106 33 0.3659683849811515 1106 34 0.3858465034866754 1106 35 0.3188061854913613 1106 36 0.3251397033403265 1106 37 0.3770595024183834 1106 38 0.32608350178612966 1106 39 0.5084551700757203 1106 40 0.3490495747983573 1106 41 0.5262956060209447 1106 42 0.5851170332736189 1106 43 0.5204149400137297 1106 44 0.5695416569032261 1106 45 0.5634447894433592 1106 46 0.5759371130207995 1106 47 0.5274258840047565 1106 48 0.42399603905366945 1106 49 0.5456653520578101 1106 50 0.45469320187945783 1107 1 0.21625443168555591 1107 2 0.135504218608979 1107 3 0.1416314515821071 1107 4 0.011517967967741072 1107 5 0.10949381264651947 1107 6 0.18394931684603222 1107 7 0.1569275443192666 1107 8 0.2048572238068047 1107 9 0.17479194333800052 1107 10 0.21723113678121564 1107 11 0.2516483672799126 1107 12 0.15779909635097766 1107 13 0.10379550135972693 1107 14 0.18590361600151795 1107 15 0.2019405140779469 1107 16 0.197637382870127 1107 17 0.14131024960361316 1107 18 0.23106836972451106 1107 19 0.1791471193366263 1107 20 0.18163373116156833 1107 21 0.1700942609526379 1107 22 0.1926394597296337 1107 23 0.22941928750277185 1107 24 0.23949011383081853 1107 25 0.2608588140363608 1107 26 0.24202306442374777 1107 27 0.2554770180836812 1107 28 0.13397353689872915 1107 29 0.19439396360140648 1107 30 0.26910327517553156 1107 31 0.3065654428804976 1107 32 0.3304863162568381 1107 33 0.2554294871911651 1107 34 0.2159296651560112 1107 35 0.22072373590970784 1107 36 0.25664068246089755 1107 37 0.21724922292396798 1107 38 0.33871288557596557 1107 39 0.34202501824844944 1107 40 0.32803281530597417 1107 41 0.25210624155921124 1107 42 0.2998012986788723 1107 43 0.2105805004013135 1107 44 0.1260776046156451 1107 45 0.2737738756822594 1107 46 0.22325788309277073 1107 47 0.22624203822260824 1107 48 0.3726227270829696 1107 49 0.41050729402266484 1107 50 0.39559755324386714 1108 1 -0.19587916523777693 1108 2 -0.1379825462089599 1108 3 -0.0957083994912189 1108 4 -0.2689353431932133 1108 5 -0.38965567419868563 1108 6 -0.38715425275537996 1108 7 -0.4584213398191963 1108 8 -0.23342018038434276 1108 9 -0.42856916407492207 1108 10 -0.2911483959745311 1108 11 -0.3955611624865867 1108 12 -0.3711034883884281 1108 13 -0.4095535569423916 1108 14 -0.41929876970566937 1108 15 -0.44594327187157035 1108 16 -0.5572057065587188 1108 17 -0.45832308565557156 1108 18 -0.3284805296532767 1108 19 -0.3278047738139662 1108 20 -0.3329097716547764 1108 21 -0.14858127466341386 1108 22 -0.35202242413502915 1108 23 -0.17758066811898487 1108 24 -0.29142121425918077 1108 25 -0.4554084903372413 1108 26 -0.3030888817797788 1108 27 -0.17583195578256294 1108 28 -0.15196990680244937 1108 29 -0.23653884188207286 1108 30 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2214 45 -0.5682240151501361 2214 46 -0.5106336523570008 2214 47 -0.56714378186782 2214 48 -0.5898577267517169 2214 49 -0.4714441708790922 2214 50 -0.47834998424234243 2215 1 -0.5704127153577261 2215 2 -0.6259347495420542 2215 3 -0.558798485488559 2215 4 -0.4858303531159862 2215 5 -0.6285784359964168 2215 6 -0.7532090152863589 2215 7 -0.8399139794353587 2215 8 -0.7783043748865666 2215 9 -0.8856838696994203 2215 10 -0.8398616239431931 2215 11 -0.9036117566572557 2215 12 -0.959490858973993 2215 13 -1.0276295358694583 2215 14 -1.0792870935147434 2215 15 -1.0871789555862748 2215 16 -0.9488486621891994 2215 17 -1.038090000192227 2215 18 -1.0844357855332163 2215 19 -1.2211122840356126 2215 20 -1.1567855895859012 2215 21 -1.260330391975259 2215 22 -1.070203409079511 2215 23 -1.0950559010450505 2215 24 -1.1719092232494503 2215 25 -1.1148773631265385 2215 26 -1.1760559094918008 2215 27 -1.1075399872485008 2215 28 -1.2342591575337067 2215 29 -1.2195093365859435 2215 30 -1.1005477312134744 2215 31 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-1.2204871226504466 2216 18 -1.3058836445807884 2216 19 -1.3174669240436927 2216 20 -1.245165560488843 2216 21 -1.228112508131602 2216 22 -1.3013378202755206 2216 23 -1.3183634659056764 2216 24 -1.4310130862763657 2216 25 -1.4917736272589934 2216 26 -1.5647720112342611 2216 27 -1.4746125354101096 2216 28 -1.467576969943714 2216 29 -1.4534286315246234 2216 30 -1.636124024966584 2216 31 -1.422824424828498 2216 32 -1.4086014144980226 2216 33 -1.3836955116791998 2216 34 -1.5607816721701369 2216 35 -1.498915617962929 2216 36 -1.3867214280656839 2216 37 -1.8222239391214146 2216 38 -1.6680254333157265 2216 39 -1.565691701638734 2216 40 -1.7397187326862067 2216 41 -1.8044829459330067 2216 42 -1.5794443097933488 2216 43 -1.5847878802843347 2216 44 -1.548717911894503 2216 45 -1.4550996999468673 2216 46 -1.484735430256837 2216 47 -1.4634618050100592 2216 48 -1.4107498193017882 2216 49 -1.2708348964309657 2216 50 -1.5047949112492631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56408.0 3 8841.0 4 14727.0 5 9055.0 6 6679.0 7 5537.0 8 4645.0 9 4336.0 10 4135.0 11 4024.0 12 4064.0 13 4320.0 14 4835.0 15 5105.0 16 5546.0 17 5819.0 18 6373.0 19 6797.0 20 7611.0 21 9271.0 22 11161.0 23 13329.0 24 15410.0 25 17835.0 26 19897.0 27 22981.0 28 26689.0 29 30815.0 30 36368.0 31 43602.0 32 53115.0 33 65341.0 34 82348.0 35 106772.0 36 138301.0 37 185411.0 38 281636.0 39 588730.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33848668688033 15.954469730348386 13.150437079117447 27.556606503653843 2 16.692218161794152 18.392748381391 35.54770933453478 29.36732412228007 3 19.420178469194408 21.798968228228667 27.302428845393788 31.47842445718314 4 13.828688886177412 14.459733002950296 32.57307691104374 39.13850119982855 5 15.711349670850156 36.013165993761284 31.750232126276696 16.52525220911187 6 36.31773874973857 32.68541201714276 15.664021121247416 15.33282811187126 7 31.933636074888117 29.75398032823137 18.514556681115298 19.797826915765214 8 29.479411813796673 32.11839458882045 17.844155275148964 20.558038322233905 9 27.954888873009427 14.24629711441717 17.00815920543328 40.79065480714013 10 16.40435958490576 27.20525987726605 30.69986516478845 25.69051537303974 11 38.156257808013244 20.529695618540703 20.775013940319266 20.539032633126787 12 25.54555497485424 22.89891311491314 27.115639190453688 24.43989271977893 13 30.276899192292582 19.095188238135705 24.27008717689486 26.357825392676848 14 24.059102342997665 18.724679777103788 24.48540543134757 32.73081244855098 15 26.154555622329063 26.148295606303417 21.708953074919872 25.988195696447647 16 25.953397622668383 25.15996254281101 23.031371699998175 25.855268134522436 17 24.856677655278265 25.295422188578765 24.370928505508825 25.476971650634145 18 25.10412797515953 24.413158520756735 25.403469819581066 25.079243684502668 19 25.699447807794336 24.276424757617097 25.457547452794998 24.566579981793566 20 26.314452377151714 23.46958044985858 25.086204288252535 25.129762884737172 21 26.466910623602963 23.40369482604375 25.118974650561682 25.010419899791604 22 26.17288793577879 23.14419569337282 25.0179055909784 25.665010779869995 23 25.59550047183074 23.151422456221983 25.28626745630284 25.966809615644436 24 25.360798176800103 23.61120022284018 25.147086515926137 25.880915084433582 25 25.586489077693958 23.442140832492647 25.300847223337918 25.670522866475476 26 25.579124447400336 23.640233167716442 25.22593012792927 25.554712256953955 27 25.406270180493816 23.78776197475945 25.094127821878548 25.71184002286818 28 25.28863638023325 23.729420932879457 25.193076273865252 25.78886641302205 29 25.32757332843712 23.70822396835266 25.33847505226568 25.625727650944537 30 25.421187948487773 23.808366329584167 25.239928080257172 25.530517641670887 31 25.79683747791054 23.406950955662516 24.977571505171074 25.81864006125587 32 25.318767749523417 23.727523789903017 24.840648723077443 26.11305973749612 33 25.407583185563787 23.59168665901742 25.067539376238656 25.933190779180137 34 25.524782238187772 23.658721789532205 25.164462190124237 25.652033782155787 35 25.622755199904873 23.658621935898392 25.267602857281428 25.451020006915304 36 25.614215697011605 23.79866137223427 24.93587958399696 25.65124334675716 37 25.61397213937302 23.31751216314389 25.268465318677237 25.800050378805857 38 25.596648287665698 23.672250594106032 24.961944360281958 25.769156757946316 39 25.73435507799972 23.745916193644774 24.89884675655169 25.62088197180381 40 25.748465211548684 23.556141213574033 25.017846983690283 25.677546591187006 41 25.46862159445017 23.748899753461345 24.994994138971045 25.78748451311744 42 25.681656771410722 23.775051440382864 24.867840192224865 25.675451595981546 43 25.305134477132444 23.425807491131582 25.17081116990073 26.098246861835232 44 25.36260752037777 23.667049749292932 24.891071741433827 26.07927098889547 45 25.453532947620022 23.844841466822956 24.8853392789358 25.81628630662123 46 25.64904625787485 23.55139758078729 24.857676500432262 25.94187966090559 47 25.41027884465978 23.83997221877506 24.778892108943282 25.97085682762188 48 25.535080016935684 24.055606191118777 24.60815373930309 25.801160052642448 49 25.574675185857615 23.77814716982313 24.445190644184734 26.201987000134526 50 25.544059470730524 23.937730303344733 24.545546030426767 25.97266419549798 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 763.0 1 502.5 2 242.0 3 359.0 4 476.0 5 493.0 6 510.0 7 472.5 8 435.0 9 521.0 10 607.0 11 1018.5 12 1430.0 13 2433.0 14 3436.0 15 4646.5 16 5857.0 17 6432.5 18 7008.0 19 6897.5 20 6787.0 21 6385.0 22 5983.0 23 5848.0 24 5713.0 25 5943.0 26 6173.0 27 6755.5 28 7338.0 29 8252.0 30 9166.0 31 10919.0 32 12672.0 33 15264.0 34 17856.0 35 22317.5 36 26779.0 37 33100.5 38 39422.0 39 48304.0 40 57186.0 41 68255.5 42 79325.0 43 91351.0 44 103377.0 45 116415.0 46 129453.0 47 142295.0 48 155137.0 49 161793.5 50 168450.0 51 171130.0 52 173810.0 53 171500.5 54 169191.0 55 165119.0 56 161047.0 57 151273.0 58 141499.0 59 131719.5 60 121940.0 61 108386.0 62 94832.0 63 82612.5 64 70393.0 65 59281.5 66 48170.0 67 39787.5 68 31405.0 69 26374.0 70 21343.0 71 17090.0 72 12837.0 73 10868.0 74 8899.0 75 6636.5 76 4374.0 77 3442.5 78 2511.0 79 2086.0 80 1661.0 81 1313.5 82 966.0 83 823.0 84 680.0 85 557.5 86 435.0 87 305.5 88 176.0 89 118.0 90 60.0 91 46.5 92 33.0 93 23.5 94 14.0 95 10.5 96 7.0 97 4.0 98 1.0 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0012513889113385742 2 0.009020428402565556 3 1.5642361391732178E-4 4 0.00495341444071519 5 0.042182234553037774 6 0.029094792188621852 7 0.026174884728831845 8 0.03404820662933704 9 0.0337875006061415 10 0.037228820112322585 11 0.040044445162834376 12 0.03373535940150239 13 0.05015983886282119 14 0.048126331881896 15 0.04906487356539993 16 0.05287118150405476 17 0.05401828800611512 18 0.051984781025189934 19 0.0502641212720994 20 0.04750063742622671 21 0.045050000808188675 22 0.04536284803602332 23 0.0452585656267451 24 0.04171296371128581 25 0.04082656323242099 26 0.04140011648345116 27 0.04145225768809027 28 0.03915804468396955 29 0.03863663263757848 30 0.03816736179582651 31 0.03467390108500633 32 0.029407639416456497 33 0.024454224975741304 34 0.021221470288116655 35 0.019917940172138972 36 0.01997008137677808 37 0.020647917037086475 38 0.014703819708228249 39 0.012201041885551099 40 0.009281134425761091 41 0.006100520942775549 42 0.005996238533497335 43 0.0056312501010235844 44 0.0048491320314369745 45 0.0019292245716469687 46 0.0028677662551508994 47 0.0014078125252558961 48 0.0013035301159776815 49 1.5642361391732178E-4 50 9.385416835039306E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1917869.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.858112674981825 #Duplication Level Percentage of deduplicated Percentage of total 1 73.31216048970596 38.01830279121523 2 12.802089795708138 13.277844302021355 3 5.269743926139904 8.198369228701926 4 2.6545035143596305 5.506301693751879 5 1.5918225317907388 4.127445610608949 6 1.0180170750791302 3.1675466510705386 7 0.6821341155088768 2.476193147505588 8 0.46467949203650094 1.9277921164625746 9 0.34801963393795365 1.62428772508743 >10 1.7401129595347065 15.37832772152648 >50 0.08375902064460228 2.9708378595334053 >100 0.032146441599874294 2.901238094940416 >500 5.068774712681356E-4 0.15682053999800427 >1k 3.041264827608809E-4 0.2686925175761981 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2235 0.11653559236840473 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.214120463910726E-5 0.0 4 0.0 0.0 0.0 5.214120463910726E-5 0.0 5 0.0 5.214120463910726E-5 0.0 5.214120463910726E-5 0.0 6 0.0 5.214120463910726E-5 0.0 5.214120463910726E-5 0.0 7 0.0 5.214120463910726E-5 0.0 1.0428240927821453E-4 0.0 8 0.0 5.214120463910726E-5 0.0 1.0428240927821453E-4 0.0 9 0.0 5.214120463910726E-5 0.0 1.0428240927821453E-4 0.0 10 0.0 5.214120463910726E-5 0.0 1.0428240927821453E-4 0.0 11 0.0 5.214120463910726E-5 0.0 1.0428240927821453E-4 0.0 12 0.0 5.214120463910726E-5 0.0 2.607060231955363E-4 0.0 13 0.0 5.214120463910726E-5 0.0 2.607060231955363E-4 0.0 14 0.0 5.214120463910726E-5 0.0 2.607060231955363E-4 0.0 15 0.0 5.214120463910726E-5 0.0 2.607060231955363E-4 0.0 16 0.0 5.214120463910726E-5 0.0 2.607060231955363E-4 0.0 17 0.0 5.214120463910726E-5 0.0 5.735532510301798E-4 0.0 18 5.214120463910726E-5 5.214120463910726E-5 0.0 5.735532510301798E-4 0.0 19 5.214120463910726E-5 5.214120463910726E-5 0.0 5.735532510301798E-4 0.0 20 5.214120463910726E-5 5.214120463910726E-5 0.0 6.778356603083943E-4 0.0 21 5.214120463910726E-5 5.214120463910726E-5 0.0 6.778356603083943E-4 0.0 22 5.214120463910726E-5 5.214120463910726E-5 0.0 9.385416835039306E-4 0.0 23 5.214120463910726E-5 5.214120463910726E-5 0.0 0.0010949652974212524 0.0 24 5.214120463910726E-5 5.214120463910726E-5 0.0 0.0017206597530905396 0.0 25 5.214120463910726E-5 5.214120463910726E-5 0.0 0.0021899305948425048 0.0 26 5.214120463910726E-5 5.214120463910726E-5 0.0 0.003024189869068221 0.0 27 5.214120463910726E-5 5.214120463910726E-5 0.0 0.0035456019154592937 0.0 28 5.214120463910726E-5 5.214120463910726E-5 0.0 0.005735532510301799 0.0 29 5.214120463910726E-5 5.214120463910726E-5 0.0 0.008186169128339839 0.0 30 5.214120463910726E-5 5.214120463910726E-5 0.0 0.012670312727303065 0.0 31 5.214120463910726E-5 5.214120463910726E-5 0.0 0.023046412450485408 0.0 32 5.214120463910726E-5 5.214120463910726E-5 0.0 0.03462175988036722 0.0 33 5.214120463910726E-5 5.214120463910726E-5 0.0 0.048126331881896 0.0 34 5.214120463910726E-5 5.214120463910726E-5 0.0 0.06674074193805729 0.0 35 5.214120463910726E-5 5.214120463910726E-5 0.0 0.09020428402565556 0.0 36 5.214120463910726E-5 5.214120463910726E-5 0.0 0.11893408778180366 0.0 37 5.214120463910726E-5 5.214120463910726E-5 0.0 0.15381655368536643 0.0 38 5.214120463910726E-5 5.214120463910726E-5 0.0 0.1924531863229449 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTAA 30 0.0057402523 29.339176 10 GGTATCA 2135 0.0 26.277468 1 GTATCAA 4155 0.0 22.292168 1 ATACCGT 165 3.6379788E-12 20.001375 6 TAATACC 135 1.0845924E-6 16.29869 4 TATAGGG 150 1.8361425E-7 16.135706 2 TATCAAC 5770 0.0 16.054354 2 CGCGTAA 110 5.614426E-5 16.003187 10 GTATATA 140 1.5866062E-6 15.714955 1 ATCAACG 5905 0.0 15.612796 3 TCAACGC 5875 0.0 15.54271 4 CAACGCA 5990 0.0 15.134111 5 ACCGTCG 175 6.3993866E-8 15.08872 8 TTAATAC 220 3.0377123E-10 15.002206 3 AACGCAG 6150 0.0 14.739224 6 GTCTTAC 225 4.274625E-10 14.667293 1 TAGGACA 410 0.0 14.489935 4 CTAGACA 305 0.0 14.428351 4 GGACCGT 185 1.265671E-7 14.271251 6 GTACATG 7670 0.0 14.055359 1 >>END_MODULE