FastQCFastQC Report
Mon 6 Feb 2017
SRR2077152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728343
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31550.019.6673641
CGGACTA600.00741847818.33113941
GGTATCA17100.016.9853381
GGACCGT1501.840217E-716.1323366
ATCAACG39050.015.9463673
TATCAAC39150.015.7927722
TCAACGC40150.015.454234
CAACGCA40350.015.3217685
AACGCAG41150.015.0221586
GTCTTAC2800.014.9311061
GTAATAC1655.410384E-714.6691563
TCGATCG1057.167526E-414.66491141
CTAGTCA1554.5624147E-614.1955464
GTGTAAC3350.013.7933861
GTATAGA4150.013.7854721
CTTACAC3550.013.6361173
ATACCGT1150.001424962813.3904776
CGCAATA1000.00876606813.19918436
GTCCTAG4100.012.8802351
GTGTAGC5650.012.8517381
Produced by FastQC (version 0.11.5)