FastQCFastQC Report
Mon 6 Feb 2017
SRR2077152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728343
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.10165806208605582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33850.021.124281
GGTATCA18400.019.6102581
TATCAAC43850.016.1568282
ATCAACG45250.015.4624513
TCAACGC45300.015.3968144
CAACGCA45450.015.1519215
AACGCAG46850.014.7452516
GTATTAT2407.2759576E-1114.6678331
ATAAGAC2703.092282E-1113.8533543
GCACCGT1751.0530985E-613.8288726
GTCTAGA3350.013.7921421
TAAGACT4350.013.6566544
GTTACGG1150.001424376613.39120725
ATTTCGG1000.00875788413.20105121
GTACATG52850.013.1968871
TAGGACC4350.013.1508524
GTATAGG2351.2358214E-813.1074261
ACCGTCG1353.2024307E-413.0399628
TACATGG53300.012.9620072
ACGCTAC2259.909854E-812.711019527
Produced by FastQC (version 0.11.5)