FastQCFastQC Report
Mon 6 Feb 2017
SRR2077151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269926
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34260.10477301321191977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG1704.4876288E-815.53222729
AATATCG7950.015.49420933
ATATCGA8300.015.370398534
GTACTAG3150.015.3685691
GTATCAA66450.015.0673811
GTACATG99150.014.7587751
TATCGAA8500.014.7499735
TCGCACG1655.432739E-714.66483613
CTTATAG7650.014.3825723
TACATGG100200.014.3179642
TACCGTG2652.3646862E-1114.1120937
TATACCG1251.6704758E-414.0819045
GCGTTAG950.006233448613.8978931
ATACCGT2401.1295924E-913.7489156
GTATAAG4700.013.5775531
CGCGCGT1952.4147994E-713.5361532
GGTATCA44050.013.3878311
ACATGGG105000.013.2660733
CGCGTAA1000.00877545613.19754510
GTATTAG4200.013.0982131
Produced by FastQC (version 0.11.5)