##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077151_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3269926 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.61084073462213 31.0 30.0 34.0 16.0 34.0 2 28.620096907391787 31.0 30.0 34.0 16.0 34.0 3 28.763048460423875 31.0 30.0 34.0 16.0 34.0 4 32.11637969788919 35.0 33.0 37.0 19.0 37.0 5 32.08698117327426 35.0 33.0 37.0 19.0 37.0 6 32.07707819687663 35.0 33.0 37.0 19.0 37.0 7 31.936409875942147 35.0 33.0 37.0 17.0 37.0 8 31.907766414285827 35.0 33.0 37.0 17.0 37.0 9 33.235792797757505 38.0 33.0 39.0 17.0 39.0 10 33.17205037667519 38.0 33.0 39.0 17.0 39.0 11 33.18974282598444 38.0 33.0 39.0 17.0 39.0 12 33.095306438127345 38.0 33.0 39.0 17.0 39.0 13 33.02894774988792 38.0 33.0 39.0 16.0 39.0 14 33.9339275567704 39.0 33.0 41.0 13.0 41.0 15 33.966342357594634 39.0 33.0 41.0 16.0 41.0 16 33.93446671270237 39.0 33.0 41.0 15.0 41.0 17 33.77255020450004 39.0 33.0 41.0 11.0 41.0 18 33.73164255093234 39.0 33.0 41.0 11.0 41.0 19 33.85724753404205 39.0 33.0 41.0 11.0 41.0 20 33.846650963966766 39.0 33.0 41.0 11.0 41.0 21 33.790025829330695 39.0 33.0 41.0 10.0 41.0 22 33.71801838940698 39.0 33.0 41.0 10.0 41.0 23 33.6710417911598 39.0 33.0 41.0 10.0 41.0 24 33.67686669361937 39.0 33.0 41.0 10.0 41.0 25 33.57620784078906 39.0 33.0 41.0 10.0 41.0 26 33.52637552042462 39.0 33.0 41.0 9.0 41.0 27 33.4700892313771 39.0 33.0 41.0 9.0 41.0 28 33.356213259871936 39.0 33.0 41.0 8.0 41.0 29 33.34475153260349 38.0 33.0 41.0 8.0 41.0 30 33.180657605095654 38.0 32.0 40.0 8.0 41.0 31 33.179594278280305 38.0 32.0 40.0 2.0 41.0 32 33.04954148809484 38.0 32.0 40.0 2.0 41.0 33 33.00865585337405 38.0 32.0 40.0 2.0 41.0 34 32.92145235090947 38.0 32.0 40.0 2.0 41.0 35 32.85096757541302 38.0 32.0 40.0 2.0 41.0 36 32.76486379202465 38.0 32.0 40.0 2.0 41.0 37 32.655468655865604 38.0 32.0 40.0 2.0 41.0 38 32.57292947913806 38.0 32.0 40.0 2.0 41.0 39 32.46345238393774 38.0 31.0 40.0 2.0 41.0 40 32.388931737293134 38.0 31.0 40.0 2.0 41.0 41 32.27410742628427 38.0 31.0 40.0 2.0 41.0 42 32.13140052710673 38.0 31.0 40.0 2.0 41.0 43 32.019832558901946 38.0 31.0 40.0 2.0 41.0 44 31.864398766210613 38.0 31.0 40.0 2.0 41.0 45 31.717502169773873 38.0 31.0 40.0 2.0 41.0 46 31.56917373665337 38.0 30.0 40.0 2.0 41.0 47 31.411022451272597 38.0 30.0 40.0 2.0 41.0 48 31.228439114524303 37.0 30.0 40.0 2.0 41.0 49 31.052121362991087 37.0 30.0 40.0 2.0 41.0 50 30.841663695141726 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.7226019953159195 1101 2 1.5822446818169027 1101 3 1.8532562720216426 1101 4 2.017379268779486 1101 5 2.1075291635936253 1101 6 1.9142371609061115 1101 7 2.0146388857940885 1101 8 2.253939783872621 1101 9 2.6645363193567704 1101 10 2.656104563746112 1101 11 2.5509440409774697 1101 12 2.7295568088305444 1101 13 2.677740819654133 1101 14 3.081395531084013 1101 15 3.094812377302695 1101 16 3.1030863620640403 1101 17 3.2362303706964184 1101 18 3.1746355866067475 1101 19 3.0772445576846295 1101 20 3.1427252156480847 1101 21 3.1508629591432538 1101 22 3.155845912578023 1101 23 3.1980482860562205 1101 24 3.219217976966206 1101 25 3.2347424447114363 1101 26 3.280756110757359 1101 27 3.2959585776259814 1101 28 3.324925328888682 1101 29 3.349638045648369 1101 30 3.529494429871633 1101 31 3.5153136936459646 1101 32 3.5501958576862833 1101 33 3.424290615313204 1101 34 3.4988413730712296 1101 35 3.440489600912997 1101 36 3.594138077412218 1101 37 3.6727371425590434 1101 38 3.6141779813668293 1101 39 3.679571366791322 1101 40 3.4538863977447747 1101 41 3.6275422211490067 1101 42 3.70177867993921 1101 43 3.591066942027936 1101 44 3.569954357228962 1101 45 3.6917047612643437 1101 46 3.553176765288569 1101 47 3.6012828881984866 1101 48 3.509443937737281 1101 49 3.496193627740542 1101 50 3.6211271481582266 1102 1 0.8157555923446118 1102 2 0.6630254373696438 1102 3 0.6980929279366421 1102 4 0.9190105273547644 1102 5 0.8820681867680662 1102 6 0.8368848738329504 1102 7 1.012578359009371 1102 8 1.1212753642379205 1102 9 1.3153223156505192 1102 10 1.3622271406417639 1102 11 1.1783929880545188 1102 12 1.2554001923868299 1102 13 1.2080468730217504 1102 14 1.4498588388415214 1102 15 1.317027507587781 1102 16 1.4677466048576946 1102 17 1.7201973786402291 1102 18 1.6515543393694614 1102 19 1.5874306942346479 1102 20 1.522633980155696 1102 21 1.5644982055384773 1102 22 1.4976587232855394 1102 23 1.4854658477563447 1102 24 1.3708542400061177 1102 25 1.5465858958690148 1102 26 1.588651777678571 1102 27 1.482680075723131 1102 28 1.6455029655277897 1102 29 1.5132843674981302 1102 30 1.6605516779936451 1102 31 1.5974792912167857 1102 32 1.6980846791561959 1102 33 1.5996569011045594 1102 34 1.6316191725441342 1102 35 1.6260955135977255 1102 36 1.684354627980774 1102 37 1.678057790914508 1102 38 1.6208634538818174 1102 39 1.7867894784246872 1102 40 1.6951783831520828 1102 41 1.59491590107789 1102 42 1.5926833197834753 1102 43 1.5282128351345392 1102 44 1.3496374778096474 1102 45 1.3616389878317214 1102 46 1.4354525595299208 1102 47 1.353031907569541 1102 48 1.4474222127820013 1102 49 1.4896492647202813 1102 50 1.5115669470739128 1103 1 0.03648399451822115 1103 2 0.1141403160996326 1103 3 0.1253152083066098 1103 4 0.03843104022853794 1103 5 0.16277181711387811 1103 6 0.16456105284953537 1103 7 0.06354667878540354 1103 8 0.3353607636583469 1103 9 0.3491769990165068 1103 10 0.3296964060066827 1103 11 0.2293815929604648 1103 12 0.28951387735689593 1103 13 0.27988854709448674 1103 14 0.2690418958487797 1103 15 0.2660488011150264 1103 16 0.057544036751366434 1103 17 0.3324915966502431 1103 18 0.21626572369508068 1103 19 0.3471655369591957 1103 20 0.25981038239748244 1103 21 0.180642470186541 1103 22 0.17698100286541063 1103 23 0.23387297679435903 1103 24 0.1772313433233066 1103 25 0.33447290352294345 1103 26 0.19077724205047275 1103 27 0.1747566862226293 1103 28 0.2957486197827137 1103 29 0.3900752848023572 1103 30 0.4461309746970983 1103 31 0.3247651168646257 1103 32 0.42489089686366555 1103 33 0.3857687601466111 1103 34 0.43646601359900217 1103 35 0.3321302021464092 1103 36 0.3775957025901917 1103 37 0.40765705351230963 1103 38 0.362508355092352 1103 39 0.4724543899171536 1103 40 0.39604764313722995 1103 41 0.4384264216919789 1103 42 0.3860820680000927 1103 43 0.3709704090116972 1103 44 0.24393774691386838 1103 45 0.1703803962802226 1103 46 0.18935136593867696 1103 47 0.2296861998630817 1103 48 0.15055705989204426 1103 49 0.17710525997045679 1103 50 0.18024752321421644 1104 1 0.2648523184029301 1104 2 0.35922253544631033 1104 3 0.33365210742831053 1104 4 0.26939101919468555 1104 5 0.27414734074059055 1104 6 0.3055119412034131 1104 7 0.358676757026533 1104 8 0.3517761066871863 1104 9 0.4264332265441624 1104 10 0.21221441968212673 1104 11 0.34165953313198116 1104 12 0.3957868580752901 1104 13 0.40219548901136903 1104 14 0.41151705132573824 1104 15 0.4328493352099798 1104 16 0.44030704443227364 1104 17 0.5870168255337589 1104 18 0.4713634262962998 1104 19 0.5424014145756075 1104 20 0.41082292308900037 1104 21 0.3921797001675742 1104 22 0.37550061355578634 1104 23 0.4406865253008263 1104 24 0.4366252910491326 1104 25 0.4090327649469572 1104 26 0.34661130142986707 1104 27 0.5116106699099348 1104 28 0.36624044653752463 1104 29 0.45906025808328366 1104 30 0.4996246739920167 1104 31 0.4888140652577917 1104 32 0.5131483118418103 1104 33 0.5747852154722963 1104 34 0.4829771832520535 1104 35 0.5548286647617715 1104 36 0.6418703983239027 1104 37 0.5868015736259338 1104 38 0.6190904567250541 1104 39 0.6636305959985265 1104 40 0.5918944878011771 1104 41 0.5944586758945931 1104 42 0.5769213428819953 1104 43 0.6578173184040139 1104 44 0.6588252713516916 1104 45 0.7563129172439069 1104 46 0.7469293106354442 1104 47 0.7358935357064986 1104 48 0.7367780517024727 1104 49 0.8056740699480187 1104 50 0.953846139745611 1105 1 0.521020105302064 1105 2 0.46224410892025247 1105 3 0.3468967020612297 1105 4 0.48292092587470137 1105 5 0.3659176498171277 1105 6 0.39595716907128065 1105 7 0.39777130668290184 1105 8 0.46582922483117173 1105 9 0.4401641278713555 1105 10 0.350456414619444 1105 11 0.4766286385676324 1105 12 0.3707514985792031 1105 13 0.44704980061560917 1105 14 0.5094004991547578 1105 15 0.538680325181339 1105 16 0.45380993609369114 1105 17 0.5502508759467659 1105 18 0.4339650416707954 1105 19 0.3480174842576318 1105 20 0.503435824414737 1105 21 0.6390736638561876 1105 22 0.6272369557004538 1105 23 0.5088437447100418 1105 24 0.6205636444492058 1105 25 0.4556675226226048 1105 26 0.5770141778930622 1105 27 0.6069345265347224 1105 28 0.6428387502997737 1105 29 0.5078901769073383 1105 30 0.571357183858133 1105 31 0.4028150076928938 1105 32 0.4944079607120173 1105 33 0.553148003538567 1105 34 0.5660144785214811 1105 35 0.5759293213394372 1105 36 0.5656075461924104 1105 37 0.6689654802521403 1105 38 0.5792720281919586 1105 39 0.626799381326208 1105 40 0.5787602075241765 1105 41 0.5933219362691986 1105 42 0.5545439630697402 1105 43 0.6237587608969264 1105 44 0.6415019179407118 1105 45 0.6677037289379548 1105 46 0.6823304745718843 1105 47 0.6385302087942328 1105 48 0.6590895483199617 1105 49 0.808075643291069 1105 50 0.727652552231131 1106 1 0.09018853809396177 1106 2 0.010792872542488396 1106 3 -0.0031714295658318292 1106 4 0.05457671789526586 1106 5 0.08036550266077569 1106 6 0.08627043513417476 1106 7 0.0909074685007063 1106 8 0.1884090191445047 1106 9 0.21672027543117167 1106 10 0.10683925546501882 1106 11 0.0992702976502855 1106 12 0.17542218551928102 1106 13 0.2043412291333837 1106 14 0.25832435661980924 1106 15 0.12847966622942408 1106 16 0.035330622912844944 1106 17 0.2586344947078487 1106 18 -0.10761338986772984 1106 19 0.1019743896164158 1106 20 0.04901675785928461 1106 21 0.18986732831198339 1106 22 0.19210803839864354 1106 23 0.20946168818198885 1106 24 0.21022811645897121 1106 25 0.17688475552819938 1106 26 0.1712684652133376 1106 27 0.26487915226673664 1106 28 0.12676721223363074 1106 29 0.2804676878366408 1106 30 0.21911684979264834 1106 31 0.11448972263622181 1106 32 0.26883622197838264 1106 33 0.2274121385749126 1106 34 0.21466893223714578 1106 35 0.14586994339584436 1106 36 0.1924643962235848 1106 37 0.23997153925791537 1106 38 0.267761076698001 1106 39 0.2649345479869254 1106 40 0.3352767437059967 1106 41 0.20380507330026632 1106 42 0.1674259337397075 1106 43 0.12679267405805206 1106 44 0.2595017275875726 1106 45 0.3341274274197268 1106 46 0.2177900059645772 1106 47 0.18258013680005902 1106 48 0.3332546300146042 1106 49 0.2179812406549182 1106 50 0.2814756128734004 1107 1 -0.08490263612779003 1107 2 -0.12563152044996784 1107 3 -0.14775115350143864 1107 4 -0.21290480178155136 1107 5 -0.3549067681588305 1107 6 -0.20024019007666638 1107 7 -0.23247784604926025 1107 8 -0.21138294521235323 1107 9 -0.17335465336770284 1107 10 -0.30712608358971494 1107 11 -0.27267754858801396 1107 12 -0.4127335317397254 1107 13 -0.23332360582823952 1107 14 -0.43510442827906815 1107 15 -0.2792700541034918 1107 16 -0.3732397206288667 1107 17 -0.2756347185725403 1107 18 -0.2567990364039403 1107 19 -0.20602416089285924 1107 20 -0.1822834428251383 1107 21 -0.14416298612422906 1107 22 -0.5001430440482011 1107 23 -0.4801050599931145 1107 24 -0.48722908039324864 1107 25 -0.5268137560094459 1107 26 -0.0841367578540968 1107 27 -0.29542788960867483 1107 28 -0.24733040108811366 1107 29 -0.20261088912300096 1107 30 -0.20554936822880165 1107 31 -0.16635457451273794 1107 32 -0.10461085928792357 1107 33 -0.14249934636748662 1107 34 -0.1615889999893838 1107 35 0.011075859384909847 1107 36 -0.18745431689171 1107 37 -0.21529930759165694 1107 38 -0.19685048078553535 1107 39 -0.11847390835487914 1107 40 -0.05233077663667274 1107 41 -0.06738416709514894 1107 42 -0.11342257011543921 1107 43 -0.26597265368063816 1107 44 -0.07776673398947764 1107 45 -0.005919493479929372 1107 46 -0.08032537914833071 1107 47 -0.050996733271016836 1107 48 -0.1732990501329077 1107 49 -0.16667043758772593 1107 50 -0.05337497421276183 1108 1 0.004721592427028298 1108 2 -0.07940438522373938 1108 3 -0.025762499467166577 1108 4 -0.09449416182256698 1108 5 -0.10776946625819761 1108 6 -0.16886130912134334 1108 7 -0.07292021806641458 1108 8 -0.08164003826627209 1108 9 -0.23755947529013355 1108 10 -0.2008205769561684 1108 11 -0.18598853628296297 1108 12 -0.3137508024113913 1108 13 -0.1569734583320752 1108 14 -0.3041209159667986 1108 15 -0.17879729104461717 1108 16 -0.19942803000417797 1108 17 -0.28370268658493814 1108 18 -0.16158592969787122 1108 19 -0.3128062724913647 1108 20 -0.3958668234000129 1108 21 -0.1607217056603929 1108 22 -0.4166662129149259 1108 23 -0.5721867872445543 1108 24 -0.3875118342506667 1108 25 -0.07638281062066454 1108 26 -0.2636337045818067 1108 27 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-1.8492295762469197 2216 41 -1.8121980570552552 2216 42 -1.7203011396178276 2216 43 -1.917287751219611 2216 44 -1.9082091485200081 2216 45 -1.9853479026990968 2216 46 -2.1452529532616644 2216 47 -2.0980555128582523 2216 48 -2.2328600837145025 2216 49 -2.1242541467593625 2216 50 -2.1900939882804984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 200175.0 3 9781.0 4 17425.0 5 14134.0 6 11387.0 7 9820.0 8 8672.0 9 8081.0 10 7893.0 11 7835.0 12 8027.0 13 8243.0 14 8720.0 15 8882.0 16 9558.0 17 10482.0 18 11243.0 19 12826.0 20 15498.0 21 20185.0 22 24266.0 23 28305.0 24 30099.0 25 32709.0 26 36389.0 27 40699.0 28 46859.0 29 53589.0 30 62434.0 31 74622.0 32 90492.0 33 112367.0 34 143082.0 35 184831.0 36 244766.0 37 334425.0 38 517005.0 39 804120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.274824160285014 16.63734474378516 12.79709106936257 25.290740026567253 2 16.295000474454454 18.918274455066104 38.40965928048217 26.37706578999728 3 20.11754510186395 22.99990724473706 27.576415183577357 29.306132469821634 4 13.267187854913828 15.300440391619762 35.03574623205877 36.396625521407636 5 15.065683769618124 36.489058112772774 33.16065395732583 15.284604160283275 6 34.39911851111362 34.30845440495063 16.74461699081456 14.547810093121186 7 30.415691251663347 30.617786123349163 20.20033858922787 18.766184035759625 8 27.777560183913053 33.86874683681419 18.854484578952814 19.499208400319944 9 26.805419939430976 16.242345870128187 18.014618695737187 38.93761549470366 10 15.661133749773215 28.590707844793588 32.08194342418339 23.6662149812498 11 36.21491216663786 22.28821091633381 22.049684785086303 19.447192131942032 12 24.540172398441065 23.98040223039068 28.620888967901188 22.85853640326707 13 28.904109169904846 20.77278707584983 24.810849677202214 25.51225407704311 14 22.746455709951853 20.255087913079795 25.753629196964155 31.244827180004194 15 24.448702195155384 27.996737129653198 22.46683890770948 25.087721767481934 16 24.529992855383476 27.044653088325514 23.96497149037925 24.460382565911765 17 23.987769425583416 27.098806285900846 25.217068829567808 23.69635545894793 18 23.77898103282202 26.73075346095615 25.967635730310402 23.522629775911426 19 24.568586044259998 26.227440905016863 26.034014939791923 23.169958110931212 20 24.816623859264073 25.636793903548543 25.479498503092508 24.06708373409488 21 25.097576419942346 25.532381782796865 25.41675605879712 23.953285738463673 22 24.763500640428237 25.20443969032961 25.52641364777824 24.50564602146391 23 24.636315735039695 25.61321900735661 25.527052258786654 24.223412998817043 24 24.378025252364502 25.677399452057475 25.67831758631074 24.26625770926728 25 24.533465716478887 25.415819377807374 25.40550500048358 24.645209905230153 26 24.337531925573053 25.669492083069994 25.798831780348998 24.194144211007952 27 24.314703133406496 25.576255324306015 25.71743091638353 24.39161062590396 28 23.81906539035123 25.82313407434072 25.816338649681782 24.54146188562627 29 24.165470710788025 26.003034345894005 25.47421219180225 24.35728275151572 30 24.326840560832558 25.873163846303576 25.603379783732038 24.19661580913183 31 24.394620892234258 25.495746894314912 25.873967321461354 24.235664891989476 32 24.398565601985148 25.780917733235015 25.357574496599117 24.46294216818072 33 24.155147233637877 25.82620320282923 25.388160296287865 24.630489267245032 34 24.412682424746063 25.74418648767231 25.45676176302667 24.386369324554956 35 24.67345855332508 25.782691245565104 25.52287786690493 24.020972334204885 36 24.684278084356723 25.794755128673913 25.243402603005684 24.277564183963683 37 24.446929090585854 25.424355835598433 25.86020927941896 24.268505794396756 38 24.67778507664164 25.675737281287773 25.42845979660341 24.218017845467177 39 24.540325001308048 25.697127469185947 25.382064253068858 24.380483276437147 40 24.47697029224306 25.428022410094947 25.825002983320942 24.270004314341055 41 24.227757440956506 25.80618711881208 25.65887476614355 24.307180674087864 42 24.44122259498864 25.880041142317296 25.482354510449866 24.196381752244196 43 24.345648887096786 25.16500655620747 25.951231361616905 24.538113195078836 44 24.604148967316533 25.44487638422048 25.554215920191314 24.39675872827167 45 24.31824174802247 25.596073875166947 25.460771483652056 24.624912893158527 46 24.222797650092613 25.77810474860549 25.495975148857546 24.503122452444355 47 24.647987283858015 25.488571746719707 25.3273562245099 24.536084744912383 48 24.366047596061783 25.78090443407256 25.66463368749874 24.188414282366917 49 24.835291159468255 25.22689717237333 25.44643758438862 24.49137408376979 50 24.459813475205074 25.556827414397304 25.43832225572153 24.545036854676088 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2514.0 1 1917.0 2 1320.0 3 1280.5 4 1241.0 5 1194.0 6 1147.0 7 1066.5 8 986.0 9 1082.0 10 1178.0 11 1472.0 12 1766.0 13 2445.0 14 3124.0 15 4086.0 16 5048.0 17 5506.0 18 5964.0 19 6252.5 20 6541.0 21 7292.5 22 8044.0 23 10018.0 24 11992.0 25 15496.0 26 19000.0 27 24189.5 28 29379.0 29 36585.0 30 43791.0 31 52629.0 32 61467.0 33 71838.5 34 82210.0 35 97296.5 36 112383.0 37 120855.5 38 129328.0 39 142334.5 40 155341.0 41 171397.5 42 187454.0 43 193671.5 44 199889.0 45 211777.0 46 223665.0 47 239344.0 48 255023.0 49 256518.5 50 258014.0 51 253565.5 52 249117.0 53 242891.5 54 236666.0 55 233630.5 56 230595.0 57 215110.0 58 199625.0 59 180982.0 60 162339.0 61 142928.0 62 123517.0 63 106203.0 64 88889.0 65 75006.5 66 61124.0 67 51205.5 68 41287.0 69 33960.0 70 26633.0 71 21626.5 72 16620.0 73 13795.0 74 10970.0 75 8568.5 76 6167.0 77 4878.5 78 3590.0 79 2861.0 80 2132.0 81 1653.0 82 1174.0 83 941.0 84 708.0 85 540.0 86 372.0 87 305.5 88 239.0 89 187.0 90 135.0 91 99.0 92 63.0 93 54.0 94 45.0 95 36.0 96 27.0 97 28.0 98 29.0 99 41.5 100 54.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08397743557499465 2 0.09223450316612669 3 0.09984935438905958 4 0.10055273422089675 5 0.09128646947973745 6 0.05562817017877469 7 0.05073509308773348 8 0.056882021183354003 9 0.05868634336067544 10 0.04364013130572374 11 0.04651481409671045 12 0.05501653554239454 13 0.04972589593770624 14 0.05018462191499135 15 0.05009287671953433 16 0.05293697777870202 17 0.052692323924149964 18 0.053640357610539195 19 0.06143869922438611 20 0.06694341095180747 21 0.07605676703387171 22 0.07893144982485843 23 0.05639271347424987 24 0.07431360832018828 25 0.08079693546581787 26 0.07831981518847826 27 0.07229521402013378 28 0.09241799355704074 29 0.08150031529765504 30 0.0763931660838808 31 0.052784069119606994 32 0.050643347892276466 33 0.05360977587872019 34 0.04868611705585998 35 0.05538351632422263 36 0.0544660643696524 37 0.056729112524258954 38 0.04688179487853853 39 0.051774871969579736 40 0.05370152107417722 41 0.04162173700566924 42 0.038899962873777574 43 0.03877763594650154 44 0.036973313769180095 45 0.029113808691695164 46 0.02633087109616548 47 0.0308875491371976 48 0.025444000873414262 49 0.025199347018862204 50 0.026606106682536546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3269926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.661370352732305 #Duplication Level Percentage of deduplicated Percentage of total 1 76.38298438844852 40.98815613915506 2 12.371810249286561 13.277765834413913 3 4.581416507328549 7.375352638196358 4 2.2024242740616855 4.727404185770869 5 1.20633274664096 3.2366734143064666 6 0.7722745505353685 2.486478640216098 7 0.47498739168121573 1.7841932036518828 8 0.34531974650625 1.4824264645906815 9 0.2576254474959823 1.2442081095333268 >10 1.2559116788573068 12.060221058719025 >50 0.08746165826219894 3.263404060256931 >100 0.056591693942504497 5.765565813349174 >500 0.003644750215460421 1.3316980888547407 >1k 0.0012149167374815157 0.9764523489855076 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3426 0.10477301321191977 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.116346363801505E-5 2 0.0 0.0 0.0 0.0 6.116346363801505E-5 3 0.0 0.0 0.0 0.0 6.116346363801505E-5 4 0.0 0.0 0.0 0.0 6.116346363801505E-5 5 0.0 0.0 0.0 0.0 6.116346363801505E-5 6 0.0 0.0 0.0 0.0 6.116346363801505E-5 7 0.0 0.0 0.0 0.0 6.116346363801505E-5 8 0.0 0.0 0.0 0.0 6.116346363801505E-5 9 0.0 0.0 0.0 3.0581731819007524E-5 6.116346363801505E-5 10 0.0 0.0 0.0 3.0581731819007524E-5 6.116346363801505E-5 11 0.0 0.0 0.0 6.116346363801505E-5 6.116346363801505E-5 12 0.0 0.0 0.0 6.116346363801505E-5 2.446538545520602E-4 13 0.0 0.0 0.0 6.116346363801505E-5 3.363990500090828E-4 14 0.0 0.0 0.0 6.116346363801505E-5 3.363990500090828E-4 15 0.0 0.0 0.0 6.116346363801505E-5 5.198894409231279E-4 16 0.0 0.0 0.0 6.116346363801505E-5 5.504711727421355E-4 17 0.0 0.0 0.0 9.174519545702258E-5 5.81052904561143E-4 18 0.0 0.0 0.0 9.174519545702258E-5 5.81052904561143E-4 19 0.0 0.0 0.0 9.174519545702258E-5 6.116346363801505E-4 20 0.0 0.0 0.0 1.223269272760301E-4 6.727981000181656E-4 21 0.0 0.0 0.0 1.8349039091404516E-4 7.033798318371731E-4 22 0.0 0.0 0.0 2.1407212273305266E-4 7.033798318371731E-4 23 0.0 0.0 0.0 2.446538545520602E-4 7.033798318371731E-4 24 0.0 0.0 0.0 3.9756251364709785E-4 7.033798318371731E-4 25 0.0 0.0 0.0 4.893077091041204E-4 7.033798318371731E-4 26 0.0 0.0 0.0 7.645432954751882E-4 7.645432954751882E-4 27 0.0 0.0 0.0 0.0011926875409412935 7.645432954751882E-4 28 0.0 0.0 0.0 0.002263048154606557 7.645432954751882E-4 29 0.0 0.0 0.0 0.004006206868289986 7.645432954751882E-4 30 0.0 0.0 0.0 0.006575072341086618 7.645432954751882E-4 31 0.0 0.0 0.0 0.01360887065945835 8.257067591132031E-4 32 0.0 0.0 0.0 0.021957683446047403 8.257067591132031E-4 33 0.0 0.0 0.0 0.03113220299174966 8.257067591132031E-4 34 0.0 0.0 0.0 0.04281442454661054 9.174519545702257E-4 35 0.0 0.0 0.0 0.059603795315245664 9.174519545702257E-4 36 0.0 0.0 0.0 0.0792678488748675 9.174519545702257E-4 37 0.0 0.0 0.0 0.10382497952553055 9.174519545702257E-4 38 0.0 0.0 0.0 0.13147086508991335 0.0010091971500272483 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGCG 170 4.4876288E-8 15.532227 29 AATATCG 795 0.0 15.494209 33 ATATCGA 830 0.0 15.3703985 34 GTACTAG 315 0.0 15.368569 1 GTATCAA 6645 0.0 15.067381 1 GTACATG 9915 0.0 14.758775 1 TATCGAA 850 0.0 14.74997 35 TCGCACG 165 5.432739E-7 14.664836 13 CTTATAG 765 0.0 14.382572 3 TACATGG 10020 0.0 14.317964 2 TACCGTG 265 2.3646862E-11 14.112093 7 TATACCG 125 1.6704758E-4 14.081904 5 GCGTTAG 95 0.0062334486 13.897893 1 ATACCGT 240 1.1295924E-9 13.748915 6 GTATAAG 470 0.0 13.577553 1 CGCGCGT 195 2.4147994E-7 13.53615 32 GGTATCA 4405 0.0 13.387831 1 ACATGGG 10500 0.0 13.266073 3 CGCGTAA 100 0.008775456 13.197545 10 GTATTAG 420 0.0 13.098213 1 >>END_MODULE